6FPA

Crystal structure of anti-mTFP1 DARPin 1238_G01 in space group P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99986
Spacegroup name	P 21 21 21
Unit cell lengths	37.990, 89.156, 103.838
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.578 - 1.580
R-factor	0.1471
Rwork	0.145
R-free	0.18000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ydy
RMSD bond length	0.006
RMSD bond angle	0.859
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2131: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.578	1.680
High resolution limit [Å]	1.580	1.580
Rmeas	0.078	1.190
Number of reflections	92607	4720
<I/σ(I)>	14.1	1.2
Completeness [%]	99.2	97
Redundancy	4.6	4.2
CC(1/2)	0.999	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.2 M NaF, 20% PEG3350