6FP9
Crystal structure of anti-mTFP1 DARPin 1238_E11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-08 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00003 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.746, 91.597, 54.410 |
Unit cell angles | 90.00, 116.93, 90.00 |
Refinement procedure
Resolution | 48.510 - 2.100 |
R-factor | 0.1643 |
Rwork | 0.162 |
R-free | 0.20490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ydy |
RMSD bond length | 0.008 |
RMSD bond angle | 0.812 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10pre_2131: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.510 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.273 | 1.540 |
Number of reflections | 25698 | 1843 |
<I/σ(I)> | 7.7 | 2.1 |
Completeness [%] | 99.4 | 85.8 |
Redundancy | 3.7 | 3.1 |
CC(1/2) | 0.975 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M (NH4)2SO4, 0.1 M MES pH 6.5, 20% PEG8000 |