6FP8

mTFP1/DARPin 1238_E11 complex in space group C2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99986
Spacegroup name	C 1 2 1
Unit cell lengths	135.953, 75.012, 48.295
Unit cell angles	90.00, 90.73, 90.00

Refinement procedure

Resolution	39.607 - 1.855
R-factor	0.1498
Rwork	0.148
R-free	0.17640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hqk
RMSD bond length	0.012
RMSD bond angle	1.301
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2131: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.607	1.970
High resolution limit [Å]	1.855	1.855
Rmeas	0.069	0.730
Number of reflections	78111	12129
<I/σ(I)>	11.2	1.7
Completeness [%]	96.6	93.1
Redundancy	2.4	2.3
CC(1/2)	0.998	0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	10% PEG4000, 20% Glycerol, and 0.02 M of L-glutamate, glycine, DL-alanine, L-lysin, DL-serin