6FP5
Crystal structure of ZAD-domain of CG2712 protein from D.melanogaster
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9 and 1.28268 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 97.650, 97.650, 48.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.050 - 2.000 |
| R-factor | 0.1613 |
| Rwork | 0.160 |
| R-free | 0.19230 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.269 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 97.650 | 97.650 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.182 | 0.058 | 0.759 |
| Rmeas | 0.195 | 0.063 | 0.809 |
| Rpim | 0.067 | 0.023 | 0.274 |
| Total number of observations | 119795 | ||
| Number of reflections | 16137 | 223 | 1173 |
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 98.9 | 96 | 99.7 |
| Redundancy | 7.4 | 6.9 | 7.8 |
| CC(1/2) | 0.993 | 0.990 | 0.947 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.1M MES pH 6.5; 1.3M Magnesium sulfate |
