6FOW
The crystal structure of EncM complexed with dioxygen under 10 bar of oxygen pressure.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 166.210, 174.852, 132.176 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.713 - 2.040 |
| R-factor | 0.1936 |
| Rwork | 0.192 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xlo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.755 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11rc1_2513: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.040 | 49.040 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 117300 | |
| <I/σ(I)> | 7.1 | |
| Completeness [%] | 96.4 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 295 | 0.1M HEPES-Na, 0.2M calcium acetate, 20% PEG 3350, pH 7.5 |
