6FOH
X-ray structure of homo sapiens Fumarylacetoacetate hydrolase domain containing protein 1 (FAHD1) at 1.56A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-22 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.966 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.090, 77.820, 119.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 65.290 - 1.560 |
R-factor | 0.16335 |
Rwork | 0.162 |
R-free | 0.18917 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.534 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.290 | 1.600 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmeas | 0.071 | 0.830 |
Number of reflections | 58013 | |
<I/σ(I)> | 13.36 | |
Completeness [%] | 97.9 | |
Redundancy | 4.77 | |
CC(1/2) | 0.999 | 0.518 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG4000 50mM MgCl2 100mM HEPES pH7.5 |