6FOH
X-ray structure of homo sapiens Fumarylacetoacetate hydrolase domain containing protein 1 (FAHD1) at 1.56A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-22 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.090, 77.820, 119.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.290 - 1.560 |
| R-factor | 0.16335 |
| Rwork | 0.162 |
| R-free | 0.18917 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.534 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.290 | 1.600 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmeas | 0.071 | 0.830 |
| Number of reflections | 58013 | |
| <I/σ(I)> | 13.36 | |
| Completeness [%] | 97.9 | |
| Redundancy | 4.77 | |
| CC(1/2) | 0.999 | 0.518 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG4000 50mM MgCl2 100mM HEPES pH7.5 |
