6FOG
X-ray structure of homo sapiens Fumarylacetoacetate hydrolase domain containing protein 1 (FAHD1) in complex with inhibitor oxalate at 1.94A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-22 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.966 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 75.984, 116.679, 125.753 |
Unit cell angles | 90.00, 89.94, 90.00 |
Refinement procedure
Resolution | 85.530 - 1.940 |
R-factor | 0.1824 |
Rwork | 0.181 |
R-free | 0.20826 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.624 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.050 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmeas | 0.136 | 1.005 |
Number of reflections | 154275 | |
<I/σ(I)> | 6.43 | |
Completeness [%] | 94.3 | |
Redundancy | 2.29 | |
CC(1/2) | 0.991 | 0.583 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG4000, 50mM MgCl2, 100mM HEPES pH7.5 |