6FOD
Vitamin D nuclear receptor complex 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0721 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 65.930, 65.930, 264.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.830 - 2.500 |
R-factor | 0.1895 |
Rwork | 0.187 |
R-free | 0.24360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hc4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 12779 | |
<I/σ(I)> | 23.8 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.6 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | BisTris pH 6.5, 1.6 M lithium sulfate and 50 mM magnesium sulfate |