6FOA
Human Xylosyltransferase 1 apo structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92819 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.283, 86.777, 153.257 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.629 - 1.869 |
R-factor | 0.1966 |
Rwork | 0.195 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gak |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.29) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 86.810 | 86.810 | 1.920 |
High resolution limit [Å] | 1.869 | 8.360 | 1.870 |
Rmerge | 0.119 | 0.042 | 1.891 |
Rmeas | 0.129 | 0.045 | 2.065 |
Rpim | 0.048 | 0.017 | 0.822 |
Number of reflections | 74977 | 969 | 5466 |
<I/σ(I)> | 9.5 | ||
Completeness [%] | 100.0 | 99.9 | 100 |
Redundancy | 7.1 | 6.5 | 6.1 |
CC(1/2) | 0.998 | 0.998 | 0.501 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 55-65% precipitant mix 2 (PEG8000 and ethylene glycol), 100 mM Bicine/Tris buffer at pH 7.5 100 mM of a sodium nitrate/sodium phosphate/ammonium sulphate mix. |