6FO8
Vitamin D nuclear receptor complex 4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0721 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 66.690, 66.690, 264.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.160 - 2.300 |
Rwork | 0.198 |
R-free | 0.23540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hc4 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 16402 | |
<I/σ(I)> | 28.08 | |
Completeness [%] | 100.0 | 99.8 |
Redundancy | 8 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | BisTris pH 6.5, 1.6 M lithium sulfate and 50 mM magnesium sulfate |