6FNN
Caldiarchaeum Subterraneum Ubiquitin:Rpn11-homolog complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.283 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 84.674, 94.571, 170.855 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.900 - 1.850 |
| R-factor | 0.17409 |
| Rwork | 0.173 |
| R-free | 0.20058 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6fjv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.385 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.900 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.074 | 0.779 |
| Number of reflections | 58917 | 9241 |
| <I/σ(I)> | 19.8 | 2.66 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 12.9 | 12.9 |
| CC(1/2) | 1.000 | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 100 mM sodium acetate, pH 4.6, 1.5 M ammonium sulfate |
