6FN6

Modifying region (DH-ER-KR) of an insect fatty acid synthase (FAS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	75.448, 174.149, 194.454
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	129.729 - 2.700
R-factor	0.2223
Rwork	0.221
R-free	0.25460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vz9
RMSD bond length	0.003
RMSD bond angle	0.692
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_1992)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	129.729	2.797
High resolution limit [Å]	2.700	2.700
Rmeas	0.240	1.810
Number of reflections	68241	6989
<I/σ(I)>	15.57	2.33
Completeness [%]	96.0	100
Redundancy	24.06	20.87
CC(1/2)	0.998	0.832

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1 M Bis-Tris propane pH 6.5, 0.35 M Na Br, 17.5% PEG 3350, 10 mM NADP+