6FN3
X-ray structure of animal-like Cryptochrome from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-19 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.894 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 151.800, 50.130, 65.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.900 - 1.900 |
| R-factor | 0.16499 |
| Rwork | 0.164 |
| R-free | 0.20628 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.416 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.900 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 | 0.211 |
| Number of reflections | 38187 | 3803 |
| <I/σ(I)> | 13.69 | 4.35 |
| Completeness [%] | 95.5 | 96.6 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 278 | .1 M 2-(N-morpholino)ethanesulfonic acid (MES), pH 6.0, 31 % PEG 5000 |
