6FMS
IMISX-EP of Se-LspA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97858 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.750, 110.130, 85.990 |
| Unit cell angles | 90.00, 97.08, 90.00 |
Refinement procedure
| Resolution | 46.268 - 3.000 |
| R-factor | 0.2301 |
| Rwork | 0.228 |
| R-free | 0.27090 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXCD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.270 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmeas | 0.310 | 3.460 |
| Number of reflections | 41032 | 3031 |
| <I/σ(I)> | 9.14 | 1.45 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 22.7 | 22.7 |
| CC(1/2) | 0.990 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 35 % PEG 400, 100 mM MES, pH 5.6, and 100 mM ammonium phosphate monobasic |
