6FMC
Neuropilin1-b1 domain in complex with EG01377, 0.9 Angstrom structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.813010 |
| Spacegroup name | P 41 |
| Unit cell lengths | 43.440, 43.440, 91.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.440 - 0.900 |
| R-factor | 0.11015 |
| Rwork | 0.109 |
| R-free | 0.13207 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kex |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.647 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.440 | 0.950 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmeas | 0.057 | 0.478 |
| Number of reflections | 117690 | |
| <I/σ(I)> | 10.55 | |
| Completeness [%] | 94.5 | 70.7 |
| Redundancy | 3.3 | |
| CC(1/2) | 0.998 | 0.795 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 16 % w/v PEG3350 and 200 mM ammonium chloride |
