6FM9

Crystal structure of human UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase (DPAGT1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-01-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91741
Spacegroup name	P 65 2 2
Unit cell lengths	103.799, 103.799, 241.099
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.600
R-factor	0.25
Rwork	0.249
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j72
RMSD bond length	0.009
RMSD bond angle	1.030
Data reduction software	XDS (3-NOV-2014)
Data scaling software	Aimless (0.5.1)
Phasing software	MR-Rosetta
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.060	3.690
High resolution limit [Å]	3.600	3.600
Rmerge	0.055	1.132
Rmeas	0.061	1.230
Rpim	0.024	0.475
Number of reflections	9509	681
<I/σ(I)>	17.8	1.8
Completeness [%]	99.6	100
Redundancy	6.2	6.6
CC(1/2)	0.999	0.666

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.05M ADA pH 6.5 -- 24% PEG400