6FM5
Crystal structure of self-complemented CsuA/B major subunit from archaic chaperone-usher Csu pili of Acinetobacter baumannii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.8726 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 49.214, 92.018, 34.111 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.009 - 1.470 |
R-factor | 0.1979 |
Rwork | 0.197 |
R-free | 0.21910 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.370 |
Data reduction software | XDS |
Data scaling software | SCALA (0.5.9) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.210 | 49.210 | 1.520 |
High resolution limit [Å] | 1.470 | 5.690 | 1.470 |
Rmerge | 0.061 | 0.031 | 0.741 |
Rmeas | 0.070 | 0.036 | 0.852 |
Rpim | 0.033 | 0.017 | 0.412 |
Total number of observations | 116430 | 2187 | 10608 |
Number of reflections | 27069 | 546 | 2591 |
<I/σ(I)> | 12.1 | 33.9 | 1.8 |
Completeness [%] | 99.7 | 99.2 | 99.5 |
Redundancy | 4.3 | 4 | 4.1 |
CC(1/2) | 0.999 | 0.998 | 0.633 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 28-30% PEG, 0.2 M ammonium sulfate | |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 28-30% PEG, 0.2 M ammonium sulfate |