6FLZ
Structure of AcmJRL, a mannose binding jacalin related lectin from Ananas comosus, in complex with methyl-mannose.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 85.740, 85.740, 204.190 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.870 - 1.895 |
R-factor | 0.1592 |
Rwork | 0.159 |
R-free | 0.17250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6flw |
RMSD bond length | 0.005 |
RMSD bond angle | 0.818 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.900 | 2.000 |
High resolution limit [Å] | 1.895 | 1.895 |
Rmerge | 0.090 | 0.930 |
Number of reflections | 66445 | |
<I/σ(I)> | 19.9 | 2.9 |
Completeness [%] | 99.7 | 92.8 |
Redundancy | 9.5 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2M ammonium citrate, 0.1M Bis-Tris propane pH 7.0; protein solution 17mg/ml in H2O |