6FLU
Photorhabdus asymbiotica lectin (PHL) in complex with synthetic C-fucoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.3 |
| Synchrotron site | BESSY |
| Beamline | 14.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-22 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.8943 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.940, 81.940, 224.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.930 - 1.780 |
| R-factor | 0.18599 |
| Rwork | 0.184 |
| R-free | 0.22218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.712 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.930 | 1.870 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.242 | 1.325 |
| Number of reflections | 85234 | 132122 |
| <I/σ(I)> | 9 | 1.7 |
| Completeness [%] | 100.0 | 99.5 |
| Redundancy | 11 | 10.8 |
| CC(1/2) | 0.991 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 4.7 M NaCl, 100 mM HEPES |
