6FLO
Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma brucei at 2.1 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-25 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.460, 71.550, 122.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.084 - 2.139 |
R-factor | 0.223883743113 |
Rwork | 0.220 |
R-free | 0.27474 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.853 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.090 | 2.220 |
High resolution limit [Å] | 2.130 | 2.140 |
Number of reflections | 30014 | 30009 |
<I/σ(I)> | 9.2 | |
Completeness [%] | 89.0 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 32% PEG 3350, 50 mM Tris pH 8.0, 4% MPD, 0.2 M ammonium sulfate |