6FLL
SPECTROSCOPIC AND STRUCTURAL STUDY OF QW, A EGFP MUTANT SHOWING PHOTOSWITCHING PROPERTIES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-10 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9600 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.450, 62.710, 66.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.500 - 1.790 |
R-factor | 0.20371 |
Rwork | 0.202 |
R-free | 0.23216 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o24 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.663 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.130 | 1.820 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.183 | 0.766 |
Number of reflections | 20947 | |
<I/σ(I)> | 7.4 | 1.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | PEG 3350, 0.1 M AMMONIUM ACETATE, 0.2 M NH4F, PH 5.0 |