6FLK
Crystal structure of Cep120 C2C domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.400, 40.954, 69.132 |
| Unit cell angles | 102.47, 98.07, 89.92 |
Refinement procedure
| Resolution | 39.970 - 1.600 |
| R-factor | 0.1998 |
| Rwork | 0.197 |
| R-free | 0.24640 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.394 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((dev_2863: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.970 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.082 | 0.693 |
| Rmeas | 0.116 | 0.979 |
| Number of reflections | 42539 | 3744 |
| <I/σ(I)> | 6.87 | 1.08 |
| Completeness [%] | 93.8 | 83.09 |
| Redundancy | 1.8 | 1.6 |
| CC(1/2) | 0.994 | 0.439 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.03M magnesium chloride hexahydrate; 0.03M calcium chloride dehydrate, 0.1M Tris(Base)/BICINE pH 8.5, 20% v/v Ethylene glycol and 10 % w/v PEG 8000 |
