6FLJ
Crystal structure of Cep120 C2A_K76A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-17 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.842, 51.367, 66.757 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.710 - 1.751 |
| R-factor | 0.2117 |
| Rwork | 0.210 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4icw |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.243 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2863: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.710 | 1.814 |
| High resolution limit [Å] | 1.750 | 1.751 |
| Rmerge | 0.069 | 1.925 |
| Rmeas | 0.072 | |
| Rpim | 0.020 | 0.567 |
| Number of reflections | 14678 | 1425 |
| <I/σ(I)> | 24.44 | 1.29 |
| Completeness [%] | 99.9 | 99.65 |
| Redundancy | 12.7 | 12.2 |
| CC(1/2) | 1.000 | 0.712 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350 and 0.2 M NaSCN |
