6FKQ
THE CRYSTAL STRUCTURE OF A FRAGMENT OF NETRIN-1 IN COMPLEX WITH A FRAGMENT OF DRAXIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 130.794, 130.794, 183.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.010 - 3.070 |
| R-factor | 0.237 |
| Rwork | 0.235 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4urt |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.881 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (3.3.22) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.130 | 3.230 |
| High resolution limit [Å] | 3.070 | 3.070 |
| Rmerge | 0.587 | 2.897 |
| Number of reflections | 18063 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 32.9 | 18.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 1.6 M AMMONIUM SULPHATE AND 0.1 M SODIUM CITRATE, AT PH 4 TO PH 5, VAPOR DIFFUSION, TEMPERATURE 298K |
