6FKP

Crystal structure of BAZ2A PHD zinc finger in complex with H3 10-mer AA mutant peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	72.719, 72.719, 99.894
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.720 - 2.000
R-factor	0.19525
Rwork	0.194
R-free	0.22255
RMSD bond length	0.007
RMSD bond angle	1.264
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.720	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.090	0.580
Number of reflections	18751
<I/σ(I)>	14
Completeness [%]	99.9
Redundancy	9.7
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	2.2-2.4 M sodium/potassium phosphate