6FK8

Crystal structure of N2C/D282C stabilized opsin bound to RS08

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	242.183, 242.183, 110.967
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.770 - 2.870
R-factor	0.2294
Rwork	0.229
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j4q
RMSD bond length	0.009
RMSD bond angle	1.253
Data reduction software	XDS ((VERSION Oct 15)
Data scaling software	Aimless
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.770	2.970
High resolution limit [Å]	2.870	2.870
Rmerge	0.193	3.531
Number of reflections	28482
<I/σ(I)>	12.28	0.72
Completeness [%]	100.0	100
Redundancy	11.5	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	see publication