6FK6
Crystal structure of N2C/D282C stabilized opsin bound to RS01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000020 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 244.209, 244.209, 111.752 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.790 - 2.360 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INHOUSE MODEL |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.772 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.790 | 2.420 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.114 | 5.370 |
| Number of reflections | 52217 | |
| <I/σ(I)> | 15.39 | 0.55 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | AMMONIUM SULPHATE, SODIUM ACETATE, D(+)-TREHALOSE, PH 6.0 |
