6FK4
Structure of 3' phosphatase NExo (WT) from Neisseria bound to DNA substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-12-19 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.91730 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 59.156, 59.156, 292.091 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.566 - 2.297 |
R-factor | 0.2001 |
Rwork | 0.199 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jc4 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.616 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.680 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.078 | 0.582 |
Rmeas | 0.086 | 0.630 |
Rpim | 0.239 | |
Number of reflections | 15625 | |
<I/σ(I)> | 13.79 | 2.2 |
Completeness [%] | 99.0 | 96.9 |
Redundancy | 6.3 | 6.8 |
CC(1/2) | 0.998 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 277 | 100 mM imidazole pH = 6.6, 55 mM (NH4)2SO4, 10% (w/v) PEG 8000, 16 - 19 % (v/v) MPD, |