6FJU
Rpn11 homolog from Caldiarchaeum Subterraneum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.452, 65.614, 99.708 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.800 - 1.650 |
| R-factor | 0.16698 |
| Rwork | 0.166 |
| R-free | 0.19025 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ocl |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.890 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.800 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.055 | 0.973 |
| Number of reflections | 51685 | |
| <I/σ(I)> | 18.8 | 1.77 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 6.86 | 6.29 |
| CC(1/2) | 0.999 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 100 mM HEPES pH 7.0, 15% PEG 4000 |
