6FJF

Tubulin-FcMaytansine complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-25
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00036
Spacegroup name	P 21 21 21
Unit cell lengths	104.373, 156.739, 180.956
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.547 - 2.402
R-factor	0.2033
Rwork	0.201
R-free	0.25750
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4i4t
RMSD bond length	0.002
RMSD bond angle	0.545
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2863: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.216	2.586
Rmeas	0.234	2.814
Number of reflections	116072	8494
<I/σ(I)>	9.96	0.75
Completeness [%]	100.0	99.8
Redundancy	6.8	6.5
CC(1/2)	0.994	0.277

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.7	294	10 % PEG 4k, 16 % glycerol, 30 mM MgCl2, 30 mM CaCl2, and 100 mM 2-(N-morpholino)ethanesulfonic acid/imidazole