6FJ7
Caldiarchaeum Subterraneum Ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-22 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 29.450, 42.800, 51.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.890 - 1.050 |
R-factor | 0.15544 |
Rwork | 0.153 |
R-free | 0.19512 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ubq |
RMSD bond length | 0.020 |
RMSD bond angle | 2.337 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.900 | 1.110 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmerge | 0.051 | 0.627 |
Number of reflections | 30589 | 4406 |
<I/σ(I)> | 22.39 | 2.18 |
Completeness [%] | 98.2 | 89 |
Redundancy | 11 | 5.1 |
CC(1/2) | 0.999 | 0.862 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 100 mM sodium acetate pH 4.5, 200 mM lithium sulfate, 50% PEG 400 |