6FIH
Crystal structure of the ANX2 ectodomain from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-08 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.999894 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.566, 41.766, 93.371 |
| Unit cell angles | 90.00, 117.37, 90.00 |
Refinement procedure
| Resolution | 44.290 - 1.080 |
| R-factor | 0.16029 |
| Rwork | 0.159 |
| R-free | 0.18269 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.714 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.290 | 1.150 |
| High resolution limit [Å] | 1.080 | 1.080 |
| Rmeas | 0.040 | 1.290 |
| Number of reflections | 175203 | 25949 |
| <I/σ(I)> | 18.94 | 1.17 |
| Completeness [%] | 96.4 | 88.7 |
| Redundancy | 6.46 | 5.5 |
| CC(1/2) | 1.000 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES pH 7.5, 28% PEG 3350 |
