6FIG
Crystal structure of the ANX1 ectodomain from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-24 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.991876 |
Spacegroup name | P 1 |
Unit cell lengths | 53.991, 68.745, 70.046 |
Unit cell angles | 88.54, 74.93, 71.74 |
Refinement procedure
Resolution | 47.270 - 1.480 |
R-factor | 0.17221 |
Rwork | 0.171 |
R-free | 0.19214 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.482 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.270 | 1.520 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmeas | 0.060 | 2.170 |
Number of reflections | 151428 | 10966 |
<I/σ(I)> | 17.93 | 1.2 |
Completeness [%] | 98.4 | 95.6 |
Redundancy | 10.3 | 10.1 |
CC(1/2) | 1.000 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 27.5% PEG 3350, 0.1M HEPES pH 7.5 |