6FIF
Crystal structure of the BRI1 Gly644-Asp (bri1-6) mutant from Arabidopsis thaliana.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97963 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 119.684, 67.569, 151.850 |
| Unit cell angles | 90.00, 100.65, 90.00 |
Refinement procedure
| Resolution | 19.890 - 2.540 |
| R-factor | 0.2071 |
| Rwork | 0.205 |
| R-free | 0.25447 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3riz |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.928 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.890 | 2.690 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmeas | 0.071 | 1.250 |
| Number of reflections | 39088 | 5972 |
| <I/σ(I)> | 13.9 | 1 |
| Completeness [%] | 98.5 | 95 |
| Redundancy | 5.2 | 5 |
| CC(1/2) | 0.990 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 18% [w/v] PEG 4,000, 0.2 M (NH4)2SO4, 0.1 M citric acid pH 4.0 |
