6FI0
Crystal structure of BAZ2A PHD zinc finger in complex with Fr 19
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 72.095, 72.095, 99.198 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.340 - 1.900 |
R-factor | 0.1916 |
Rwork | 0.190 |
R-free | 0.23101 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4qf2 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.898 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.340 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.045 | |
Number of reflections | 21069 | |
<I/σ(I)> | 14.3 | |
Completeness [%] | 99.3 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 2.2-2.4 M sodium/potassium phosphate |