6FHW
Structure of Hormoconis resinae Glucoamylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-19 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 137.975, 149.827, 192.349 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.580 - 3.600 |
R-factor | 0.2647 |
Rwork | 0.264 |
R-free | 0.27310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1glm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.223 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.580 | 3.760 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmeas | 0.142 | 0.675 |
Number of reflections | 38033 | |
<I/σ(I)> | 10.5 | |
Completeness [%] | 81.6 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | tacsimate |