6FHQ

Crystal structure of human BAZ2B PHD zinc finger in complex with Fr 21

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	38.339, 45.369, 65.039
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.240 - 1.950
R-factor	0.1872
Rwork	0.185
R-free	0.23884
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4qf3
RMSD bond length	0.020
RMSD bond angle	1.880
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.040	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.081
Number of reflections	8453	527
<I/σ(I)>	11.4
Completeness [%]	97.7
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	2.2 - 2.4 M sodium/ potassium phosphate