6FHO
Crystal structure of pqsL, a probable FAD-dependent monooxygenase from Pseudomonas aeruginosa - new refinement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.077, 63.257, 128.628 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.670 - 1.750 |
| R-factor | 0.18171 |
| Rwork | 0.180 |
| R-free | 0.21875 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.035 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.670 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.080 | 0.740 |
| Number of reflections | 37305 | |
| <I/σ(I)> | 2.28 | 2.28 |
| Completeness [%] | 99.5 | 95 |
| Redundancy | 5.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 31% PEG1500, 0.1M HEPES, PH 7.5. CRYSTALS WERE CRYOPROTECTED WITH 10% PEG400 IN A SOLUTION CONTAINING 35% PEG1500, 0.1M HEPES, PH 7.5 |
