6FHK

Structure of a modified protein containing a genetically encoded phosphoserine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	51.625, 110.876, 63.483
Unit cell angles	90.00, 90.41, 90.00

Refinement procedure

Resolution	55.090 - 1.657
R-factor	0.1638
Rwork	0.162
R-free	0.19440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ay9
RMSD bond length	0.010
RMSD bond angle	1.394
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.090	1.662
High resolution limit [Å]	1.657	1.657
Number of reflections	83152
<I/σ(I)>	13.2
Completeness [%]	98.3
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M Succinic acid pH 7.0, 15% w/v Polyethylene glycol 3,350