6FHF
Highly active enzymes by automated modular backbone assembly and sequence design
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2017-10-16 |
| Detector | RIGAKU |
| Wavelength(s) | 1.541 |
| Spacegroup name | H 3 |
| Unit cell lengths | 128.969, 128.969, 51.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.942 - 1.850 |
| R-factor | 0.1858 |
| Rwork | 0.184 |
| R-free | 0.22630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mmd |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.978 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1-2575_1692: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.920 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 27384 | 4010 |
| <I/σ(I)> | 12.7 | 2.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 292 | (NH4)2H2PO4 0.05M 0.05M Sodium acetate pH=4.5 |
