6FGK
Crystal structure of the small alarmone synthethase 2 from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.670, 99.920, 122.884 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.281 - 3.200 |
R-factor | 0.2601 |
Rwork | 0.257 |
R-free | 0.31920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dec |
RMSD bond length | 0.004 |
RMSD bond angle | 0.735 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.280 | 3.420 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.169 | 0.572 |
Number of reflections | 16011 | 1561 |
<I/σ(I)> | 3.7 | 0.9 |
Completeness [%] | 92.2 | 92.7 |
Redundancy | 4.1 | 4.2 |
CC(1/2) | 0.980 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M CHES pH 9.5, 30% (w/v) PEG 3000 |