6FG7
Crystal structure of the BIR2 ectodomain from Arabidopsis thaliana.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000020 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 153.770, 153.770, 110.055 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.770 - 1.900 |
| R-factor | 0.21535 |
| Rwork | 0.214 |
| R-free | 0.23267 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.336 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.770 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.221 | 2.880 |
| Number of reflections | 60341 | 9433 |
| <I/σ(I)> | 11.9 | 1 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 13.2 | 12.8 |
| CC(1/2) | 0.998 | 0.429 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 298 | 1.8 M sodium malonate pH 4.0 |
