6FF5
X-ray structure of bovine heart cytochrome c at high ionic strength
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-25 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.966 |
Spacegroup name | H 3 2 |
Unit cell lengths | 83.350, 83.350, 92.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 56.900 - 1.740 |
R-factor | 0.16289 |
Rwork | 0.161 |
R-free | 0.20255 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b4z |
RMSD bond length | 0.022 |
RMSD bond angle | 2.075 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.900 | 1.780 |
High resolution limit [Å] | 1.740 | 1.740 |
Rmerge | 0.040 | 0.800 |
Number of reflections | 12830 | 3810 |
<I/σ(I)> | 20.4 | |
Completeness [%] | 99.6 | 100 |
Redundancy | 6 | |
CC(1/2) | 0.999 | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 3.2-3.4 M ammonium sulfate and 0.4-0.5 M NaNO3 |