6FF1
CsoZ metallochaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 51.048, 51.048, 64.955 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.210 - 1.300 |
| R-factor | 0.14359 |
| Rwork | 0.142 |
| R-free | 0.17217 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.780 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.210 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 24651 | 1184 |
| <I/σ(I)> | 10 | 0.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.8 | 8.4 |
| CC(1/2) | 0.997 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM CaCl2, 100 mM sodium acetate pH 5.0, 20%w/v PEG 6000 |
