6FF1
CsoZ metallochaperone
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 51.048, 51.048, 64.955 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.210 - 1.300 |
R-factor | 0.14359 |
Rwork | 0.142 |
R-free | 0.17217 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.780 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXCD |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.210 | 1.320 |
High resolution limit [Å] | 1.300 | 1.300 |
Number of reflections | 24651 | 1184 |
<I/σ(I)> | 10 | 0.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.8 | 8.4 |
CC(1/2) | 0.997 | 0.868 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM CaCl2, 100 mM sodium acetate pH 5.0, 20%w/v PEG 6000 |