6FF0
Ryegrass mottle virus serine protease domain fused with VPg domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2016-09-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96411 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.940, 67.520, 74.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.900 - 2.100 |
| R-factor | 0.17623 |
| Rwork | 0.173 |
| R-free | 0.23481 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zyo |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.897 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.080 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.077 | 0.380 |
| Rmeas | 0.084 | 0.417 |
| Rpim | 0.034 | 0.170 |
| Number of reflections | 11945 | 1699 |
| <I/σ(I)> | 13.9 | 4.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 5.8 | 5.9 |
| CC(1/2) | 0.998 | 0.924 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 0.1 M MIB, pH 9.0, 25% PEG 1500 |
