6FEJ
Anabaena Apo-C-Terminal Domain Homolog Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-21 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 81.080, 81.080, 162.943 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 70.220 - 2.750 |
| R-factor | 0.2465 |
| Rwork | 0.241 |
| R-free | 0.29210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ui2 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.220 | 2.848 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.071 | 0.334 |
| Rpim | 0.032 | 0.142 |
| Number of reflections | 8819 | 1247 |
| <I/σ(I)> | 11.7 | 5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.6 | |
| CC(1/2) | 0.935 | 0.898 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 288 | 0.1M citric acid 25% w/v PEG 3350 |
