6FDM

Human Rio2 kinase structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	110
Detector technology	CCD
Collection date	2010-09-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9801
Spacegroup name	P 1 21 1
Unit cell lengths	66.920, 98.327, 117.069
Unit cell angles	90.00, 95.22, 90.00

Refinement procedure

Resolution	66.640 - 2.100
R-factor	0.2034
Rwork	0.202
R-free	0.23740
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XDS
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.640	2.170
High resolution limit [Å]	2.100	2.100
Rpim	0.022	0.336
Number of reflections	331294	31840
<I/σ(I)>	20.59	2.32
Completeness [%]	98.5	96.68
Redundancy	3.8	3.7
CC(1/2)	0.999	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Morpheus screen D2