6FDL
Crystal structure of the NYN domain of human MARF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 96.867, 96.867, 63.096 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.430 - 1.750 |
| R-factor | 0.1915 |
| Rwork | 0.190 |
| R-free | 0.21940 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.719 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.430 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.108 | 1.029 |
| Number of reflections | 30788 | 2956 |
| <I/σ(I)> | 18 | 1.7 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 20.1 | 15.9 |
| CC(1/2) | 0.997 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 12% (w/v) PEG3350, 0.2 M K3-Citrate and 0.1 M Bis-Tris propane, pH 6.5 |
