6FDH

Structure of H. salinarum RosR (vng0258) grown from KCl

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-16
Detector	MARRESEARCH
Wavelength(s)	0.9219
Spacegroup name	P 1 21 1
Unit cell lengths	41.069, 76.966, 42.129
Unit cell angles	90.00, 110.21, 90.00

Refinement procedure

Resolution	38.540 - 1.850
R-factor	0.1811
Rwork	0.179
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SAD derived model in NaCl
RMSD bond length	0.006
RMSD bond angle	0.777
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13rc2_2986)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.078	0.800
Rmeas	0.086	0.850
Rpim	0.036	0.827
Number of reflections	21011	1277
<I/σ(I)>	17.9	1.4
Completeness [%]	99.8	99
Redundancy	5.7	5.67
CC(1/2)	0.990	0.522

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	298	KCL, AMMONIUM SULFATE, MES PH 6.8