6FDE

Crystal Structure of the HHD2 Domain of Whirlin : 3-helix conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	110
Detector technology	PIXEL
Collection date	2016-04-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97857
Spacegroup name	P 41 21 2
Unit cell lengths	34.668, 34.668, 99.732
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.750 - 1.850
R-factor	0.219
Rwork	0.214
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.670	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.048	0.507
Rpim	0.030	0.389
Number of reflections	5620	314
<I/σ(I)>	15.6	2.4
Completeness [%]	98.7	92.7
Redundancy	5.6	4.1
CC(1/2)	0.999	0.376

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Na cit 2-propanolOH 20% w/vPEG4K 20% w:v